FAQ-Fluorometer
Q: Which nucleic acids are compatible with the FluoroVerse™?
A: You can quantify RNA, DNA, or Protein.
Q: How sensitive is the FluoroVerse™?
A: It is sensitive enough to detect any nucleic acid down to the nanogram.
Q: Can the FluoroVerse™ distinguish between DNA and RNA in a mixed sample?
A: Yes. It can distinguish between DNA and RNA if there is mixing of a sample. However, specificity also depends on the reagents used. We recommend using a DNA Quantification kit to measure DNA and an RNA Quantification kit to measure RNA.
Q: Does the FluoroVerse™ require a computer to operate?
A: No. The FluoroVerse™ is a stand-alone instrument.
Q: Are NovelBeamUSA instruments Wi-Fi compatible?
A: No. The FluoroVerse™ is USB compatible.
Q: How much training does it take to learn to use this instrument?
A: This software is designed to be easy to use right out of the box. No special training is needed, and there is no software to install.
Q: What sort of reproducibility should I expect with the FluoroVerse™?
A: Typically ± 0.75 ng/uL for dsDNA samples <= 200 ng/uL and ± 1% for samples > 200 ng/µL.
Q: How long will the standards S1/S2 last? Can I use the same S1/S2 as a standard if I have two batches of samples to measure in the same day, or just a few hours difference?
A: Standards S1/S2 will last and can be used for different samples on the same day. S1/S2 changes within a day will not significantly affect concentration readings.
Q: Since standards S1/S2 can be reused in the same day, do the standards S1/S2 need to be re-measured again with new batches of samples?
A: You can continue to test samples without recalibration, on the same day, if using the NovelBeamUSA assays and operating under the NovelBeamUSA assay testing mode.
Q: If my samples are out of the range with too high concentrations can I just dilute it (1:1) directly with the solution without adding the dye; And then measure them again? Then do a (2X read out data) as my concentration?
A: If the concentration is too high and out of range, you will need to dilute the sample in citric buffer, (around pH 6.2), Add this to 190uL working solution (with dye) because the dye concentration in the working solution will not be the same as in the standards S1/S2.
Q: What will be the minimum sample size? Can I use 1ul of sample only?
A: The minimum sample solution is 1uL, However, it is recommended you use a higher volume to be accurate. If the sample is limited, it can be diluted, but the sample volume will need to be changed accordingly on the sample settings screen on the fluorometer.
Q: Can the FluoroVerse measure the ssDNA concentrations?
A: Single strand ssDNA (linear and circular) can be detected using our newly developed assay (Release date expected to be Q4 2025). However, the dye used in the ssDNA assay can also pick up RNA. Therefore, when using this assay for ssDNA measurement, you need to avoid RNA contamination.
Q: Do I need the 0.5 ml PCR tube for the machine? Will 0.65 ml clear Eppendorf tube work?
A: We recommend using clear PCR tubes (Cat# PCR-05-C). These can be purchased on Amazon, and/or Thermo Fisher Qubit device recommended tubes. These fit the best and the tube's optics are the best material for the readings.
Q: Do you have any data comparison of your equipment vs. Invitrogen's Qubit 4? Can I use the same tubes?
A: Yes, learn more by clicking on the following link to compare devices:
https://novelbeamusa.com/blogs/news/wondering-how-fluoroverse-compares-to-the-industry-standard
Q: Does your machine have a Power button? Or can I just unplug the power cord when it's not in use?
A: Just unplug when not in use. To power on, plug it in and give it a minute to warm up.
Q: Why they are 4 samples tubes for the standard #2, and only one tube for the standard #1 inside the RNA kit?
A: We include 4 tubes of standard #2 because the samples can be used more than once. We included 4 tubes to reduce the likelihood of contamination of the RNA due to frequent use. Standard #1 does not contain RNA.
Q: My product was accidentally left out at room temperature or exposed to light. Is it ruined?
A: Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.
Q: My product arrived at room temperature or thawed, but it’s supposed to be stored at 4°C or -20°C. Is it still ok?
A: Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature or with an ice pack. These products may thaw without affecting product performance. When you receive the product, place it under the recommended storage conditions.
Some products are shipped with blue ice packs as an extra precaution against high temperatures. The blue ice packs may be thawed upon arrival without affecting product performance.
Products with recommended storage at ultra low temperature (-70°C) that also ship on dry ice should arrive frozen. If a product you received was shipped on dry ice and thawed during transit.
Q: Where can I find the expiration date or shelf life of a product?
A: Bioscience kits
The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. Kits often are functional for significantly longer than the guaranteed shelf life. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples.
Expiration date based on date of manufacture (DOM)
If your institution requires you to document the expiration date based on the date of manufacture for reagents please contact us.